The Calming Actions of Anemone Pulsatilla, Nepeta, and Rauvolfia


Pulsatilla, Nepeta, and Rauvolfia are important therapeutic agents with nerve-calming effects. Although not as strong as anxiolytic drugs, these herbal remedies are gentle and safe treatments for anxiety and insomnia. They can improve sleep, reduce mood swings and irritability, and moderate the stress-induced ‘fight-or-flight’ state. Theoretical concerns regarding adverse combination with alcohol or other psychoactive drugs have not been confirmed by clinical or scientific studies. The onset of depression must be monitored carefully when prescribing Rauvolfia to pre-disposed patients; however, this side effect does not appear to occur as commonly or severely as previously cited in the literature.


These are AARM reference reviews in which clinicians can obtain a quick overview of understanding with respect to how herbs, nutrients, and hormones can be used effectively in clinical practice. The dosages recommended are based on therapeutic results. Side effects that have not been seen in clinical experience or found in clinical studies but only been theorized have been identified and depicted as Unsubstantiated Theoretical Concerns.

Since many nutrients and herbs on the market have not gone through double-blind studies but have been used extensively in clinical settings for hundreds, and for some thousands of years, these reference reviews have attempted to give a unified opinion on how to use these herbs effectively. The opinions expressed are from different professors and experts in the field of nutritional and botanical medicine. Some information is based on personal opinions and can not be quantified as fact. However, if clinicians only restricted themselves to what is fully proven by double-blind studies, the art of healing and restoring health with nutrients and herbs would be lost.


In 2013, it was reported by NIMH that 18.1% and 9.5% of the American population had been prescribed medication for depression and anxiety, respectively. Given the cost and side effects of these medications, natural agents that restore and tone the nervous system provide a welcome alternative. Although not as strong as anxiolytic drugs, Anemone pulsatilla, Nepetacataria and Rauvolfia are gentle and safe treatments or alternatives for anxiety and insomnia.


Pulsatilla (Anemone pulsatilla, Pulsatilla chinensis), Catnip (Nepeta cataria), Indian or African Snake Root (Rauvolfia serpentina, Rauvolfia vomitoria)


Anxiety, Insomnia, Emotional lability and irritability


Adjunctive or Stand-Alone


Pulsatilla whole plant extract mg daily containing 4 mg of anemonic acid per day, Catnip whole plant, African Snake Root up to 10-40 mcg of reserpineper day


Ashwagandha (Ashwagandha), Pulsatilla (Anemone), Catnip (Nepeta) tincture dose (1:2 to 1:5) 3-5 ml. three times per day, Lavender (Lavender) 1.5 ml per day, African Snake Root (Rauvolfia) tincture dose (1:2, 0.1-1 ml per day)


A mild hypotensive affect is possible with low doses of Rauvolfia. Catnip is safe when used appropriately. Rauvolfia, Catnip and Pulsatilla are all contraindicated during pregnancy and when breastfeeding. Pulsatilla is an irritant to skin and mucous membranes and can also cause kidney and urinary tract irritation.


There are theoretical concerns that Rauvolfia at low doses, as recommended for anxiety, should not be taken with alcohol, antipsychotics, barbiturates, digoxin, monoamine oxidase inhibitors, propranolol, diuretics, ephedrine, and tricyclic antidepressants. There is some theoretical evidence these herbs may increase lithium levels in patients taking Lithium. No clinical or scientific studies have confirmed the findings.


St. John’s wort, Valerian, Chamomile and other herbs have been well discussed in the herbal literature, and have been safely and effectively used for decades in the management of anxiety. This review will focus on three other nerve-calming agents (Nepeta cataria, Anemone pulsatilla, and Rauvolfia serpentina) that are less commonly known and used. It should be noted that meditation, stress management techniques, and other anxiolytic herbs including Bacopa monnieri, Scutellaria lateriflora (Scullcap), Passiflora incarnata (Passionflower), Leonurus cardiaca (Motherwort), Verbena hastata(Blue Vervain), Polygala tenuifolia (Chinese Polygala) and Piper methysticum (Kava) may complement the herbal treatments discussed in this article.

Nepeta catariaThe Nepeta genus is in the mint or Lamiaceae family and contains about 250 species. Nepeta cataria (catnip) is best known for its effects on cats; catnip is a neuroexcitant in felines. Traditional human uses have been for reducing nervousness and irritability, promoting relaxation, and as a sleep aid. In the historical past, catnip was a popular culinary tea used in children’s formulas for head colds and influenza, especially if irritability and poor sleep accompanied the illness. Nepeta cataria has reported antimicrobial effects. Further, some Nepeta species have demonstrated antiviral activities. Nepeta is described in the herbal literature as a diaphoretic for chills and disturbed sleep at the onset of colds and flu. In A Modern Herbal,published in 1931, author Maude Grieve wrote extensively about Nepeta under the heading of ‘catnep’. Grieve recommended freshly expressed juice (succus) rather than tea, for nervous headaches and menstrual regulation, and as a treatment for hysteria and nightmares.

Nepeta contains terpene nepetalactone which acts on the central nervous system of cats. Though the effects on the central nervous system of humans has not been elucidated, a case report implicates Nepeta for depression of the CNS in a toddler. In addition, the plant contains iridoid glycosides, including nepeta glucosyl ester. Similar compounds are found in Valeriana and contribute to its sedative effects. A third psychoactive compound, caffeoyltartronic acid, is also found in Nepeta and has been shown to have relaxing properties.

Researchers have found that acute and long-term administration of Nepeta to mice increased sleep duration. Tincture of Nepeta persica had an anxiolytic activity in animal models. Although there are no documented clinical trials with this herb, herbalists and naturopathic medical practitioners report Nepeta to be a useful herbal tool for reducing symptoms of stress and treating anxiety disorders.

Anemone pulsatilla: Anemone pulsatilla is a perennial plant in the Ranunculaceae or buttercup family, with over 30 species in the genus. The plant blooms in the spring and is found most commonly in high altitude, sparse grasslands and prairies. Anemone is a genus of over 120 species on calcareous soils. In past botanical nomenclature, the plant was known as Pulsatillaanemone. The flower head of Pulsatilla frequently moves in a downward direction during rainy or inclement weather, hence, the common name windflower.

Contact with the freshly pulverized leaves and roots may irritate (and even blister or ulcerate) the skin. Thus, dried plant material is usually recommended for therapeutic use. Fresh extracts of Pulsatilla yield an oily substance with an acrid and peppery taste that has an ability to irritate the skin and mucous membranes. The oil breaks down into anemonic acid, isoanemonic acid, and anemonin. Anemonic acid is thought to be inert, while anemonin is a powerful irritant on par with cantharidin isolated from Spanish fly (Lytta vesicatoria). In hot water, it volatilizes and can irritate the eyes, upper respiratory mucous membranes, and gastric membranes. If injected, it causes tissue necrosis. Historically, Pulsatilla has been used in the treatment of nervous conditions (i.e., nervous exhaustion). The roots have been utilized for centuries as both herbal and homeopathic preparations for emotional distress (e.g., mood swings, irritability, extreme emotional sensitivity with tearfulness, sudden outbursts and/or hyper-reactivity). Doses of 2-3 drops in water was also used to treat spastic cough, asthma, pertussis and bronchitis.

From the mid 1800s to the 1920s, members of the Eclectic Physicians School recommended a dosage of 5-30 drops in a 4 ounce bottle of water to be taken by the teaspoon every few hours. However, homeopathic preparations of Pulsatilla are even more diluted than that prescribed by Eclectic physicians.

Pulsatilla contains triterpenoid saponins, including hederagenin, which is a natural pesticide. It also contains the glycoside ranunculin and proto-anemonin, common in Ranunculaceae, a precursor to the acrid oil of anemonin. In small doses, anemonin acts as a central nervous system depressant, lowering heart rate and respiration. This depressant capacity may account for the herb’s ability to treat anxiety and emotional hypersensitivity.

Pulsatilla also contains cinnamic acid (cinnamate), a natural tyrosinase inhibitor. Tyrosinase is the enzyme responsible for both synthesizing catecholamines (especially in the absence of tyrosine hydroxylase) and oxidizing excess dopamine. There is no published research on Pulastilla and catecholamines, but it is reasonable to hypothesize that perhaps the cinnamate in Pulsatilla would affect dopamine synthesis in these cells. Indeed, cinnamic acid and related compounds from Pulsatilla have been studied as neuroprotective agents.

Rauvolfia serpentina and Rauvolfia vomitoriaRauvolfia serpentina and Rauvolfia vomitoria are both members of the Apocynaceae family—with R. serpentina being native to India, Sri Lanka, Myanmar, and Malaysia and Rauvolfia vomitoria native to tropical regions of Africa. There are 85 species in the Rauvolfia genus. In India, Rauvolfia serpentina is specifically indicated for abdominal pain and colic in children, and it has also been used for insomnia, hypochondria, and “insanity.” As a treatment for anxiety, Rauvolfia serpentina was introduced into European herbal medicine at least 300 years ago.

Rauvolfia serpentina contains over 20 alkaloids including reserpine yohimbine, ajmaline, sparteine, deserpidine, rescinnamine, and serpentinine. Rauvolfia and its alkaloid, reserpine, were among the first drugs introduced into neuropharmacology in the 1930s. It was used briefly in the treatment of psychosis and schizophrenia, and reserpine’s effect on dopamine and neurotransmitters was investigated at the inception of the field of neuropharmacology.

Reserpine alters the sympathetic-parasympathetic balance within the central nervous system. Neurotransmittor studies in animal models have shown that very small amounts of reserpine trigger the release of serotonin. At larger doses, reserpine is noted to deplete stores of 5 hydroxytryptamine (serotonin) and catecholamines. Likewise, reserpine promotes parasympathetic nervous activity (mostly by reducing sympathetic tone), and via this mechanism, Rauvolfia reduces heart rate, blood pressure, and other functions that are under control of the sympathetic nervous system. Another component of Rauvolfia, raubasine, is a benzodiazepine receptor agonist. Due to these actions, Rauvolfia is a valuable therapeutic tool in treating individuals with stress and anxiety disorders.


Pulsatilla, Nepeta, and Rauvolfia are important therapeutic agents in the treatment of anxiety. They can improve sleep, reduce mood swings and irritability, and moderate the stress-induced ‘fight-or-flight’ state (sympathetic activation). It is certainly important to watch for the onset of depression when prescribing Rauvolfia to pre-disposed patients; however, this side effect does not appear to occur as commonly or severely as previously cited in the literature.


Dr. Saunders reports personal fees related to employment or seeing patients from CCNM, the Dundas Naturopathic Centre, and from Beaumont Health Systems, Troy Hospital, MI, outside the submitted work. Dr. Winston reports personal fees from Herbalist & Alchemist, Inc, outside the submitted work. Dr. Stansbury has nothing to disclose.


  1. Nostro A, Cannatelli MA, Crisafi G, et.al. The effect of Nepeta cataria extract on adherence and enzyme production of Staphylococcus aureus.Int J Antimicrob Agents. 2001 Dec; 18(6):583–5.
  2. Abad MJ, Guerra JA, Bermejo P, et.al. Search for antiviral activity in higher plant extracts. Phytother Res. 2000 Dec; 14(8):604–7. 1 .
  3. Grieve M. A Modern Herbal, 1931. Dover Publications, New York, reprinted 1971, 919 pp.
  4. Osterhoudt KC, Lee SK, Callahan J, et.al. Catnip and the alteration of human consciousness. Vet Hum Toxicol. 1997 Dec; 39(6):373–5.
  5. Massoco CO, Silva MR, Gorniak SL, Spinosa MS, Bernardi MM. Behavioral effects of acute and long-term administration of catnip (Nepeta cataria) in mice. Vet Hum Toxicol 1995; 37(6):530–3.
  6. Rabbani M, Sajjadi SE, Mohammadi A. Evaluation of the anxiolytic effect of Nepeta persica Boiss. in mice. Evid Based Complement Alternat Med 2008; 5(2):181–6.
  7. See website: http://www.ansci.cornell.edu/plants/toxicagents/ranunculoside.html .
  8. Proctor, William. American Journal of Pharmacy, vol 31, Third Series, Volume 7 , 1859, pages 440–1 Philadelphia College of Pharmacy .
  9. Felter HW, Lloyd JU. King's American Dispensatory, 1898. Reprinted 1983, Eclectic Institute, Sandy, OR, 2 Vols .
  10. Bang SC, Kim Y, Lee JH, Ahn BZ. Triterpenoid saponins from the roots of Pulsatilla koreana. J Nat Prod 2005; 68(2):268–72.
  11. Martin ML, Ortíz de Urbina AV, Montero MJ, Carrón R, San Román L. Pharmacologic effects of lactones isolated from Pulsatilla alpina subsp. apiifolia. J Ethnopharmacol. 1988 Dec; 24(2-3):185–91.
  12. Saify ZS, Noor F, Mushtaq N, Dar A. Assessment of Anemone pulsatilla for some biological activities. Pak J Pharm Sci 1998; 11(1):47–53.
  13. Kim YJ, Uyama H. Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future. Cell Mol Life Sci 2005; 62(15):1707–23.
  14. Lee HS. Tyrosinase inhibitors of Pulsatilla cernua root-derived materials. J Agric Food Chem 2002; 50(6):1400–03.
  15. Hasegawa T. Tyrosinase-expressing neuronal cell line as in vitro model of Parkinson’s disease. Int J Mol Sci 2010; 11(3):1082–9.
  16. Asanuma M, Miyazaki I, Ogawa N. Dopamine- or L-DOPA-induced neurotoxicity: the role of dopamine quinone formation and tyrosinase in a model of Parkinson’s disease. Neurotox Res 2003; 5(3):165–76.
  17. Lee JS, Jeon SM, Park EMet al. Cinnamate supplementation enhances hepatic lipid metabolism and antioxidant defense systems in high cholesterol-fed rats. J Med Food 2003; 6(3):183–91.
  18. Pletscher A, Shore PA, Brodie BB. Serotonin as a mediator of reserpine action in brain. J Pharmacol Exp Ther 1956; 116(1):84–9.
  19. Charveron M, Assie MB, Stenger A, Briley M. Benzodiazepine agonist-type activity of raubasine, a Rauvolfia serpentina alkaloid. Eur J Pharmacol 1984; 106(2):313–7.
  20. Baumeister AA, Hawkins MF, Uzelac SM. The myth of reserpine-induced depression: role in the historical development of the monoamine hypothesis. J Hist Neurosci 2003; 12(2):207–20.

DOI: http://dx.doi.org/10.14200/jrm.2013.2.0111